Development of Three-Dimensional Culture System for Maintenance of Bovine Nucleus Pulposus Cells
نویسندگان
چکیده
منابع مشابه
Nucleus pulposus explant culture model.
Many of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are heterogeneous and not well characterized. To address this, we have investigated the morphology, extracellular gene and protein expression, and apoptosis changes in NP explants cultured in vitro with or without chondrogenic reagents for differen...
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Intervertebral disc (IVD) degeneration greatly affects quality of life. The nucleus pulposus (NP) of chondrodystrophic dog breeds (CDBs) is similar to the human NP, because the cells disappear with age and are replaced by fibrochondrocyte-like cells. However, because IVD develops as early as within the first year of life, we used canines as a model to investigate in vitro the mechanisms underly...
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Objective(s): This research is to study the influences of different concentrations of simvastatin on the biological activities of nucleus pulposus-derived mesenchymal stem cells (NPMSC).Materials and Methods: NPMSC were cultured with different concentrations of simvastatin (0, 0.01, 0.1, and 1 μM) and assessed to determine the possible e...
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objective: spermatogonial stem cells (sscs) are the only cell type that can restore fertility to an infertile recipient following transplantation. much effort has been made to develop a protocol for differentiating isolated sscs in vitro. recently, three-dimensional (3d) culture system has been introduced as an appropriate microenvironment for clonal expansion and differentiation of sscs. this ...
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ژورنال
عنوان ژورنال: Global Spine Journal
سال: 2014
ISSN: 2192-5682,2192-5690
DOI: 10.1055/s-0034-1376659